Alvaro Bautista's perfect start to 2009 came to a dramatic end when the 250cc title contender broke his collarbone during the second and final day of testing at Valencia on Sunday.

Bautista had been on top of the timesheets ever since the test ban ended on January 20, sweeping all three days at Jerez and then remaining on top during a wet opening day at Valencia.

Sunday at least dawned dryer, but Bautista - the 2006 125cc world champion and a winner of six 250GPs - was caught out when the rain returned, falling from his Aspar Aprilia and fracturing his right collarbone.

Spanish reports state that the 2008 world championship runner-up should be fit to battle reigning world champion Marco Simoncelli in the 2009 season opener at Qatar on April 12.

Despite the accident, Bautista still finished the Valencia test second overall, with fellow former 125cc world champion - and 250cc rookie - Gabor Talmacsi nudging just 0.081sec ahead of the injured Spaniard.

Reigning 125cc world champion Mike di Meglio, one of a number of riders to sit out the soaking opening day, returned to claim an encouraging third with Alex Baldolini fourth and Hector Faubel the top Honda rider in fifth.

The next official 250cc test will take place at Jerez on March 25.

Valencia 250cc test - day two:

1. Gabor Talmacsi Aprilia 1min 38.089sec
2. Alvaro Bautista Aprilia 1min 38.170sec
3. Mike di Meglio Aprilia 1min 38.374sec
4. Alex Baldolini Aprilia 1min 39.246sec
5. Hector Faubel Honda 1min 39.451sec
6. Ratthapark Wilairot Honda 1min 39.726sec
7. Raffaele de Rosa Honda 1min 40.314sec