For the first time since Valencia 2007, a MotoGP race ended without a Yamaha rider on the podium at Aragon on Sunday.
The result also marked the end of world championship leader Jorge Lorenzo's run of 13 consecutive rostrums - the Spanish star losing third place to Nicky Hayden on the very last lap.
Lorenzo had battled with eventual winner Casey Stoner for the first few turns, but the Australian and Repsol Honda's Dani Pedrosa pulled clear within a few laps and Lorenzo spent the rest of the race trying to hold off Hayden.
"My start was very good, but during the race I struggled a lot with Nicky always behind me, very close!" said Lorenzo. "We can't be happy with the result and I am disappointed to finish my podium run, but the most important thing is that we did finish and we still took points for our championship."
Team manager Wilco Zeelenberg said that Lorenzo couldn't afford to take the same risks as Hayden.
"We're at a point in the championship when we can't afford to take risks but there are other riders who have nothing to lose, and this was the story today," he said.
Lorenzo lost seven points to nearest title rival Pedrosa, who finished second, but still holds a commanding 56 point lead.
"We have five more races to go and, although this is a complicated moment for us and we have some things to address, I am confident that we can improve again. Now we go to Motegi and I am looking forward to a good result at the home of Yamaha."
Aragon also marked a 2010 low for team-mate Valentino Rossi, who sunk as low as ninth early in the race before moving up the order to sixth position - his worst result since Le Mans 2009.
"This was a very difficult race for us; we had a lot of problems all weekend and I was very slow today," said Rossi. "I had to try to arrive to the end but I couldn't do better than sixth.
"All the Yamahas have suffered here but my shoulder problems made it even worse for me and this, combined with the problems with our package at this track, made it extra hard. We're very sad this afternoon and we hope for better in Japan."
"We ran into troubles from the start here and unfortunately we couldn't find a way to put a competitive package together," added Rossi's crew chief jerry Burgess, who looks set to join the Italian at Ducati next year.
"Valentino's shoulder is causing him problems too and this, together with the set-up issues, meant we couldn't finish any higher up the order today. We're disappointed but we have some ideas and we hope for a better weekend in Japan."
Rossi remains fourth in the championship, having lost one championship place to Stoner but gained a position from Andrea Dovizioso, who crashed from sixth on the final lap.