Seventh fastest Dovizioso, making the switch from Honda, spent some laps on the 800cc Yamaha before going out on the more powerful 2012 bike. His best lap time was 1.673sec behind former team-mate Pedrosa.
Sixth on the timesheets (+1.671sec) was lone factory Ducati rider Valentino Rossi. The Italian's team-mate Nicky Hayden was unable to ride due to wrist fractures in Sunday's turn one pile-up, and Rossi pushed on with the development of the GP12, riding with an 'experimental' aluminium twin-spar chassis alongside test rider Franco Battaini.
Satellite Desmosedici riders Héctor Barberá (Pramac Racing) and Karel Abraham (Cardion AB Motoracing) both tried the GP12 for the first time on Tuesday, finishing eighth and tenth.
Between them on the timesheet was new Moto2 World Champion Stefan Bradl. The German rode the 800cc LCR Honda as a 'gift' from HRC for winning the 600cc title, but with LCR yet to sign a rider, there may be the potential for a more long term association.
Interest was also raised by presence of three privateer 'CRT' machines. Iván Silva rode Inmotec's prototype with Carmelo Morales on a Suter housing a BMW engine under the Laglisse team banner, whilst Gianluca Nannelli and Federico Sandi shared testing duties of Italian factory Gapam's machine.
Silva was quickest of the quartet, with a best lap time 4.9sec from Pedrosa.
Testing concludes on Wednesday evening.