Ben Spies cut a frustrated figure at Assen on Friday after he was hampered set-up changes and then a slower rider in qualifying.
The Texan had been fastest on his works Yamaha YZR-M1 on day one at the Dutch TT, but will start tomorrow's race from the second row of the grid in sixth.
The American, who claimed his maiden MotoGP success at Assen in 2011, went the 'wrong direction' with set-up changes in the morning before reverting back to Thursday's settings.
"Qualifying was a frustrating experience," Spies said.
"We tried a setting this morning and went backwards so the first bit of qualifying we tried a modified setting from yesterday which again wasn't working so well.
"We went back to yesterday's set-up with a minor change but only got one lap in, which turned out to be my fastest lap,” he added.
"Unfortunately one of the CRT bikes got in the way on my qualifying lap. I know they are out there trying to do their thing as well so it's difficult."
"Ben was on form for a best lap until T9 when he caught a slower CRT rider who closed the door on him and he lost his lap," confirmed team director Massimo Meregalli.