Having skipped Monday's official Brno test, Ducati instead took its factory MotoGP riders Andrea Dovizioso and Andrea Iannone to Misano. on Tuesday.
The venue was chosen to prepare for Ducati's all-important home event at the circuit, next month.
Austria winner Iannone did a total of 59 laps in perfect weather conditions, setting a best of 1m 33.115s.
Dovizioso completed 93 laps, with his quickest a 1m 33.195s, but ended the day with a crash at Turn 4 in which he twisted his right knee.
The Italian has ten days to fully recover before the British MotoGP at Silverstone.
The official Misano race lap record is a 1m 33.273s by Jorge Lorenzo (Yamaha). The pole record, also set by the Spaniard last season, is a 1m 32.146s.
Aprilia are also testing at Misano, with Sam Lowes, plus KTM.