The Factory Yamaha team concluded testing on Wednesday, but Repsol Honda and LCR Honda continued on Thursday.
The test marked the first ever laps for full time MotoGP competitors at the circuit, American CRT wild-cards having been the only previous grand prix bikes to test at the track.
COTA, which impressed on its F1 debut late last year, will host its first MotoGP race from April 19-21, as round two of this year's world championship.
The final official MotoGP test will be held at Jerez, Spain from March 23-25.CLICK HERE
for pictures from the test...
1. Marc Marquez
ESP Repsol Honda (RC213V) 2m 3.281s
2. Dani Pedrosa
ESP Repsol Honda (RC213V) 2m 3.898s
3. Stefan Bradl
GER LCR Honda (RC213V) 2m 4.225s
4. Blake Young
USA Attack Performance (Attack-Kawasaki CRT) 2m 12.186s
(42)Fastest Day 2 time:Marc Marquez
ESP Repsol Honda 2m 3.853sFastest Day 1 time:Marc Marquez
ESP Repsol Honda 2m 4.619s