Isle of Man TT titan Michael Dunlop made up for lost time as the Northern Ireland rider scaled the Superbike summit during Wednesday practice with a searing lap of 131.574mph.
Dunlop had missed Superbike practice on Tuesday evening after his Hawk Racing BMW developed a problem on his first lap.
However, the 27-year-old made amends on the S1000RR to shoot to the top of the times on Wednesday evening to snatch the top spot away from Tyco BMW's Ian Hutchinson, who lapped at 130.691mph.
Yorkshireman Hutchinson actually lapped faster on his Superstock machine with a blistering speed of 130.745mph as the 11-time winner continues to show impressive form this week.
Behind Dunlop and Hutchinson, Mountain king John McGuinness produced his first 130mph lap of TT 2016 on the Honda Racing Fireblade, clocking a speed of 130.497mph as he finished ahead of team-mate Conor Cummins (129.764mph).
Dean Harrison, who is back on Kawasaki machinery this year after riding for Ulster's Mar-Train Yamaha team in 2015, slotted into fifth on the Superbike time sheets at 129.742mph, followed by Michael Rutter on the Bathams SMT BMW (129.163mph).
Flying Kiwi Bruce Anstey was solid again on the Valvoline Padgetts Honda RC213V-S, posting two laps and setting his quickest time from a standing start of 128.833mph.
Two nights of practice remain ahead of Saturday's six-lap RST Superbike race, which was won last year by Anstey, who was celebrating his first victory in the class.