Michel Fabrizio has taken over from Ducati Xerox team-mate Noriyuki Haga at the top of the timesheets following second qualification at Brno as not every rider improved their best times.
Haga held sway for the majority of the session with his Friday best of 1min 59.277secs before Max Biaggi usurped him with just under five minutes remaining, the Aprilia man proving the first person to go below 1min 59secs this weekend.
However, even he would be beaten by Fabrizio, the Italian setting a fine marker of 1min 58.695secs to go three tenths up on his countryman.
Haga remained third without improving his time – in fact he would be just ninth fastest in the session - ahead of Luca Scassa and Lorenzo Lanzi, who in turn made it four Ducatis and four Italians inside the top five.
Cal Crutchlow was another to make do with his provisional lap, ahead of sole BMW entrant Ruben Xaus, Jakub Smrz, Jonathan Rea and James Toseland.
With the field down to 21 now following the withdrawal of the injured Troy Corser, Leon Haslam was still only 13th fastest as he trailed his Suzuki team-mate Sylvain Guintoli, while Shane Byrne, Tom Sykes and Leon Camier in 15th, 17th and 18th respectively suggests Superpole could be a tough experience for the British contingent.
Meanwhile, Broc Parkes and Roger Lee Hayden are the two riders who will not progress to Superpole after Matteo Baiocco leapfrogged both to make it into the deciding session.