Sykes made perfect use of his qualifying tyres to claim pole with a record-breaking lap of 1m 58.010, beating Cal Crutchlow's 2010 pole time by just 0.008s.
But Englishman Sykes was a confident 0.460s clear of Althea Ducati's reigning champion Checa, with Laverty salvaging Aprilia honour in third (+0731s).
Giugliano provided a last-gasp surprise when he used Melandri, just ahead of him, as a marker to pinch the final front row start from his fellow Italian by 0.096s.
Joining Melandri on row two will be Honda's Jonathan Rea (+1.550s), Melandri's B
Row three will be headed by ParkinGO Aprilia's Chaz Davies (+1.738s) with Smrz eighth and the last of the riders to turn a lap in the final session. The unlucky Badovini and Salom will line-up eleventh and twelfth.
Fixi Crescent Suzuki team-mates Leon Camier (13th) and John Hopkins (16th), plus Kawasaki's Loris Baz (15th) joined Biaggi in going no further than Superpole 1 and will thus form row four of Sunday's grid.
Biaggi holds a 48-point lead over Melandri heading into round 9 of 14.