The session was red-flagged at the very end when Dupasquier fell in front of Sasaki on the exit of Turn 9. Sasaki was launched into the air, but it was Dupasquier who required lengthy medical treatment at the track (which is why the following MotoGP FP4 session is delayed). Alcoba was also involved.

The arrival of an air ambulance underlined the seriousness of the incident for Dupasquier.

Italian Moto2 Grand Prix, Mugello - Qualifying Results
PosRiderNatTeamBikeTime
1Tatsuki SuzukiJPNSIC58 Squadra Corse(Honda)1m 56.001s
2Pedro AcostaSPARed Bull KTM Ajo(KTM)1m 56.597s
3Gabriel RodrigoARGIndonesian Racing Gresini Moto3(Honda)1m 56.685s
4Dennis FoggiaITALeopard Racing(Honda)1m 56.980s
5Jaume MasiaSPARed Bull KTM Ajo(KTM)1m 56.985s
6Jeremy AlcobaSPAIndonesian Racing Gresini Moto3(Honda)1m 57.107s
7Romano FenatiITASterilgarda Max Racing Team(Husqvarna)1m 57.110s
8Niccolò AntonelliITAAvintia Esponsorama Moto3(KTM)1m 57.137s
9John McPheeGBRPetronas Sprinta Racing(Honda)1m 57.152s
10Ayumu SasakiJPNRed Bull KTM Tech 3(KTM)1m 57.206s
11Jason DupasquierSWICarXpert PruestelGP(KTM)1m 57.213s
12Kaito TobaJPNCIP Green Power(KTM)1m 57.298s
13Deniz ÖncüTURRed Bull KTM Tech 3(KTM)1m 57.574s
14Stefano NepaITABOE Owlride(KTM)1m 57.776s
15Sergio GarciaSPAValresa GASGAS Aspar Team(GASGAS)1m 58.456s
16Darryn BinderRSAPetronas Sprinta Racing(Honda)1m 58.472s
17Andrea MignoITARivacold Snipers Team(Honda)2m 1.176s
18Filip SalacCZERivacold Snipers Team(Honda)2m 1.325s
19Alberto SurraITATeam Bardahl VR46 Riders Academy(KTM)1m 57.698s
20Xavier ArtigasSPALeopard Racing(Honda)1m 57.794s
21Riccardo RossiITABOE Owlride(KTM)1m 57.832s
22Andi Farid IzdiharINAHonda Team Asia(Honda)1m 57.964s
23Elia BartoliniITATeam Bardahl VR46 Riders Academy(KTM)1m 57.977s
24Lorenzo FellonFRASIC58 Squadra Corse(Honda)1m 58.138s
25Ryusei YamanakaJPNCarXpert PruestelGP(KTM)1m 58.280s
26Adrian FernandezSPASterilgarda Max Racing Team(Husqvarna)1m 58.290s
27Carlos TataySPAAvintia Esponsorama Moto3(KTM)1m 58.452s
28Maximilian KoflerAUTCIP Green Power(KTM)1m 59.219s
29Izan GuevaraSPAValresa GASGAS Aspar Team(GASGAS)2m 0.834s
30Takuma MatsuyamaJPNHonda Team Asia(Honda)No Time